Persistent Link:
http://hdl.handle.net/10150/191904
Title:
Isolation and concentration of Cryptosporidium from water
Author:
Cifrino, Andrew Charles,1955-
Issue Date:
1986
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
A method was developed for the concentration and detection of Cryotosooridium oocysts in water. Oocysts were concentrated from 100 gallon volumes of tapwater using spun polypropylene cartridge filters. The filter was backflushed and washed with 0.1% Tween 80 in deionized water. Centrifugation of the eluate resulted in a suspension containing both oocysts and sediment. Density gradient centrifugation was used to remove the sediment. Membrane filtration of the final suspension was followed by labelling the oocysts with a fluorescein linked monoclonal antibody. Enumeration of the oocysts was accomplished by fluorescence microscopic observation of the membrane filter. The recovery efficiency using this technique averaged 51%. Furthermore, the detection of as few as one oocyst per gallon of water was possible. This method can now be used for the investigation of waterborne outbreaks and to study the occurrence of Cryotosooridium in water.
Type:
Thesis-Reproduction (electronic); text
LCSH Subjects:
Hydrology.; Waterborne infection.; Water -- Microbiology.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Nutrition and Food Science; Graduate College
Degree Grantor:
University of Arizona
Committee Chair:
Berry, James W.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleIsolation and concentration of Cryptosporidium from wateren_US
dc.creatorCifrino, Andrew Charles,1955-en_US
dc.contributor.authorCifrino, Andrew Charles,1955-en_US
dc.date.issued1986en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractA method was developed for the concentration and detection of Cryotosooridium oocysts in water. Oocysts were concentrated from 100 gallon volumes of tapwater using spun polypropylene cartridge filters. The filter was backflushed and washed with 0.1% Tween 80 in deionized water. Centrifugation of the eluate resulted in a suspension containing both oocysts and sediment. Density gradient centrifugation was used to remove the sediment. Membrane filtration of the final suspension was followed by labelling the oocysts with a fluorescein linked monoclonal antibody. Enumeration of the oocysts was accomplished by fluorescence microscopic observation of the membrane filter. The recovery efficiency using this technique averaged 51%. Furthermore, the detection of as few as one oocyst per gallon of water was possible. This method can now be used for the investigation of waterborne outbreaks and to study the occurrence of Cryotosooridium in water.en_US
dc.description.notehydrology collectionen_US
dc.typeThesis-Reproduction (electronic)en_US
dc.typetexten_US
dc.subject.lcshHydrology.en_US
dc.subject.lcshWaterborne infection.en_US
dc.subject.lcshWater -- Microbiology.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineNutrition and Food Scienceen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.chairBerry, James W.en_US
dc.identifier.oclc213360785en_US
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