Development of a microporous filter method for concentration of rotavirus from tap water

Persistent Link:
http://hdl.handle.net/10150/191796
Title:
Development of a microporous filter method for concentration of rotavirus from tap water
Author:
Toranzos Soria, Gary Antonio.
Issue Date:
1983
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
An adsorption-elution method was developed for the concentration of the simian rotavirus SAll from tap water. Negatively and positively charged microporous filters were compared for their suitability for rotamirus adsorption efficiency from water and sewage. The positively charged filters were far superior to the negatively charged filters in their adsorption efficiency at ambient pH. Both, the Zeta-Plus (50S) and the Virozorb 1 MDS were equally efficient in their adsorption capacities, but the elution efficiency of the 1 MDS exceeded that of the Zeta-Plus. Tryptose phosphate broth was found to be the best eluent for rotavirus. For small volumes of seeded tap water the percent recovery of rotavirus was 45.5 ± 0.7% for Zeta-Plus and 78.5 ± 3.5% for 1 MDS. However, with large volumes of input water, the percent recoveries were reduced to 13.7 ± 0.57% for Zeta-Plus and 19.5 ± 0.7% for 1 MDS.
Type:
Thesis-Reproduction (electronic); text
LCSH Subjects:
Hydrology.; Water -- Purification -- Filtration.; Water -- Purification -- Virus removal.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Microbiology; Graduate College
Degree Grantor:
University of Arizona
Committee Chair:
Gerba, Charles P.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleDevelopment of a microporous filter method for concentration of rotavirus from tap wateren_US
dc.creatorToranzos Soria, Gary Antonio.en_US
dc.contributor.authorToranzos Soria, Gary Antonio.en_US
dc.date.issued1983en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractAn adsorption-elution method was developed for the concentration of the simian rotavirus SAll from tap water. Negatively and positively charged microporous filters were compared for their suitability for rotamirus adsorption efficiency from water and sewage. The positively charged filters were far superior to the negatively charged filters in their adsorption efficiency at ambient pH. Both, the Zeta-Plus (50S) and the Virozorb 1 MDS were equally efficient in their adsorption capacities, but the elution efficiency of the 1 MDS exceeded that of the Zeta-Plus. Tryptose phosphate broth was found to be the best eluent for rotavirus. For small volumes of seeded tap water the percent recovery of rotavirus was 45.5 ± 0.7% for Zeta-Plus and 78.5 ± 3.5% for 1 MDS. However, with large volumes of input water, the percent recoveries were reduced to 13.7 ± 0.57% for Zeta-Plus and 19.5 ± 0.7% for 1 MDS.en_US
dc.description.notehydrology collectionen_US
dc.typeThesis-Reproduction (electronic)en_US
dc.typetexten_US
dc.subject.lcshHydrology.en_US
dc.subject.lcshWater -- Purification -- Filtration.en_US
dc.subject.lcshWater -- Purification -- Virus removal.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineMicrobiologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.chairGerba, Charles P.en_US
dc.identifier.oclc213096260en_US
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