Persistent Link:
http://hdl.handle.net/10150/191514
Title:
Quantification of a bacteriophage in wastewater.
Author:
Brainard-Smith, Warren B.,1943-
Issue Date:
1969
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
An investigation was conducted to estimate qualitatively and quantitatively the content of bacteriophage in wastewater before and after tertiary treatment through Tucson's pilot filter. Preliminary studies on methods to eliminate or reduce bacteria in wastewater, prior to assaying for bacteriophage, included membrane filtration and centrifugation. Membrane filtration was superior to centrifugation but serum pretreatment was necessary to eliminate loss of bacteriophage by adsorption. Five enteric bacteria, including Escherichia coli, Aerobacter cloacae, Proteus vulgaris, Pseudomonas aeruginosa, and Salmonella paratyphi, were isolated from pilot filter influent and subsequently used to assay wastewater filtrates for bacteriophage. The concentration of bacteriophage capable of causing lysis of these bacteria was determined by using a modification of the most probable number method customarily used to estimate coliforms. The concentrations of all five bacteriophages were found to be reduced after tertiary treatment through the pilot filter. A modification of the bacteriophage assay procedure was investigated in an effort to concentrate bacteriophages present in wastewater using an aluminum hydroxide adsorption method recently devised to adsorb human enteroviruses. This procedure yielded good recovery of bacteriophage and with slight modification might be used for direct plaque enumeration of bacteriophages in water rather than the indirect most probable number method used in this study.
Type:
Thesis-Reproduction (electronic); text
LCSH Subjects:
Hydrology.; Sewage -- Microbiology.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Microbiology and Medical Technology; Graduate College
Degree Grantor:
University of Arizona
Committee Chair:
Ludovici, P. P.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleQuantification of a bacteriophage in wastewater.en_US
dc.creatorBrainard-Smith, Warren B.,1943-en_US
dc.contributor.authorBrainard-Smith, Warren B.,1943-en_US
dc.date.issued1969en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractAn investigation was conducted to estimate qualitatively and quantitatively the content of bacteriophage in wastewater before and after tertiary treatment through Tucson's pilot filter. Preliminary studies on methods to eliminate or reduce bacteria in wastewater, prior to assaying for bacteriophage, included membrane filtration and centrifugation. Membrane filtration was superior to centrifugation but serum pretreatment was necessary to eliminate loss of bacteriophage by adsorption. Five enteric bacteria, including Escherichia coli, Aerobacter cloacae, Proteus vulgaris, Pseudomonas aeruginosa, and Salmonella paratyphi, were isolated from pilot filter influent and subsequently used to assay wastewater filtrates for bacteriophage. The concentration of bacteriophage capable of causing lysis of these bacteria was determined by using a modification of the most probable number method customarily used to estimate coliforms. The concentrations of all five bacteriophages were found to be reduced after tertiary treatment through the pilot filter. A modification of the bacteriophage assay procedure was investigated in an effort to concentrate bacteriophages present in wastewater using an aluminum hydroxide adsorption method recently devised to adsorb human enteroviruses. This procedure yielded good recovery of bacteriophage and with slight modification might be used for direct plaque enumeration of bacteriophages in water rather than the indirect most probable number method used in this study.en_US
dc.description.notehydrology collectionen_US
dc.typeThesis-Reproduction (electronic)en_US
dc.typetexten_US
dc.subject.lcshHydrology.en_US
dc.subject.lcshSewage -- Microbiology.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineMicrobiology and Medical Technologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.chairLudovici, P. P.en_US
dc.identifier.oclc225865027en_US
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