Isolation and characterization of coliforms : opportunistic pathogens and standard plate count bacteria from groundwater

Persistent Link:
http://hdl.handle.net/10150/191068
Title:
Isolation and characterization of coliforms : opportunistic pathogens and standard plate count bacteria from groundwater
Author:
Franzblau, Scott Gary.
Issue Date:
1982
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The bacterial flora of groundwater obtained from wells and distribution sites was investigated from both an ecological and a public health perspective. A majority of the isolates were oxidase positive, non-fermentative, gramnegative bacilli. Extensive heterogeneity of groundwater microflora, as determined by biochemical characterization and antibiograms, was observed both within and among welldistribution (N-D) systems. Of the unique isolates (sorts) found in the W-D systems, 75% were resistant to 2 or more antibiotics at clinically significant concentrations. Community diversity within W-D systems was evaluated by rarefaction which failed to reveal a general trend. Standard plate counts in 2 of 3 wells were significantly higher on Standard Methods Agar diluted ten-fold than on the same medium at the standard concentration. Pseudomonas aeruginosa, Flavobacterium sp., Aeromonas hydrophila and Yersinia enterocolitica were detected in 21, 18, 7 and 3% respectively of water samples analyzed over a 12 month period. A selective medium was developed for the isolation of Flavobacterium sp. and was effective in suppressing 98% of the background flora when used in a membrane filtration (MF) procedure. Yersinia Selective Agar was employed in an MF procedure for the isolation of Y. enterocolitica. The use of anaerobic incubation in this procedure effectively suppressed background growth both in the presence and absence of an antimicrobic supplement. Anaerobic incubation of m-endo LES Agar (AN-MF) was effective in suppressing non-coliform growth in the total coliform MF test and markedly reduced the frequency of overgrown plates. The AN-MF appeared to obviate the need for selective chemical agents and thus has potential value in the isolation of stressed coliforms.
Type:
Dissertation-Reproduction (electronic); text
Keywords:
Hydrology.; Groundwater analysis.; Bacterial growth.; Water -- Microbiology.; Water -- Purification -- Biological treatment.
Degree Name:
Ph. D.
Degree Level:
doctoral
Degree Program:
Microbiology; Graduate College
Degree Grantor:
University of Arizona
Committee Chair:
Sinclair, Norval A.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleIsolation and characterization of coliforms : opportunistic pathogens and standard plate count bacteria from groundwateren_US
dc.creatorFranzblau, Scott Gary.en_US
dc.contributor.authorFranzblau, Scott Gary.en_US
dc.date.issued1982en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe bacterial flora of groundwater obtained from wells and distribution sites was investigated from both an ecological and a public health perspective. A majority of the isolates were oxidase positive, non-fermentative, gramnegative bacilli. Extensive heterogeneity of groundwater microflora, as determined by biochemical characterization and antibiograms, was observed both within and among welldistribution (N-D) systems. Of the unique isolates (sorts) found in the W-D systems, 75% were resistant to 2 or more antibiotics at clinically significant concentrations. Community diversity within W-D systems was evaluated by rarefaction which failed to reveal a general trend. Standard plate counts in 2 of 3 wells were significantly higher on Standard Methods Agar diluted ten-fold than on the same medium at the standard concentration. Pseudomonas aeruginosa, Flavobacterium sp., Aeromonas hydrophila and Yersinia enterocolitica were detected in 21, 18, 7 and 3% respectively of water samples analyzed over a 12 month period. A selective medium was developed for the isolation of Flavobacterium sp. and was effective in suppressing 98% of the background flora when used in a membrane filtration (MF) procedure. Yersinia Selective Agar was employed in an MF procedure for the isolation of Y. enterocolitica. The use of anaerobic incubation in this procedure effectively suppressed background growth both in the presence and absence of an antimicrobic supplement. Anaerobic incubation of m-endo LES Agar (AN-MF) was effective in suppressing non-coliform growth in the total coliform MF test and markedly reduced the frequency of overgrown plates. The AN-MF appeared to obviate the need for selective chemical agents and thus has potential value in the isolation of stressed coliforms.en_US
dc.description.notehydrology collectionen_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.typetexten_US
dc.subjectHydrology.en_US
dc.subjectGroundwater analysis.en_US
dc.subjectBacterial growth.en_US
dc.subjectWater -- Microbiology.en_US
dc.subjectWater -- Purification -- Biological treatment.en_US
thesis.degree.namePh. D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineMicrobiologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.chairSinclair, Norval A.en_US
dc.contributor.committeememberRemers, William A.en_US
dc.identifier.oclc212909566en_US
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.