VITELLOGENIN OF THE TOBACCO HORNWORM, MANDUCA SEXTA: PROPERTIES AND ENDOCYTOTIC INCORPORATION INTO FOLLICLES.

Persistent Link:
http://hdl.handle.net/10150/188160
Title:
VITELLOGENIN OF THE TOBACCO HORNWORM, MANDUCA SEXTA: PROPERTIES AND ENDOCYTOTIC INCORPORATION INTO FOLLICLES.
Author:
OSIR, ELLIE ONYANGO.
Issue Date:
1986
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Manduca sexta vitellogenin is a phosphoglycolipoprotein (Mᵣ ∼ 500,000) that contains two copies of the apoproteins (apovitellogenin-I, Mᵣ 180,000 and apovitellogenin-II Mᵣ 45,000), 13 percent lipids, 3 percent carbohydrates and 0.6 percent phosphorus. The two apoproteins are immunologically distinct and apovitellogenin-II is not completely accessible to the aqueous environment in the intact molecule. The carbohydrate moiety located on apovitellogenin-I has a high mannose structure (Man₉ GlcNAc₂). Follicle membranes bind ¹²⁵I-labeled vitellogenin with high affinity and specificity (K(D) ≃ 1.3 x 10⁻⁸ M). Total binding sites were estimated at 4 x 10¹⁴ sites/g of follicle membrane protein. The binding was sensitive to pH and calcium. Competition studies showed that binding of vitellogenin was blocked by vitellin and deglycosylated vitellogenin but not by lipophorin, microvitellogenin or apovitellogenin-II. These results suggest that the uptake of vitellogenin involves binding to specific receptors on follicle membranes and the carbohydrate moiety and apovitellogenin-II are not involved in the interaction with the receptors.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Tobacco hornworm -- Eggs.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Biochemistry; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Law, John H.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleVITELLOGENIN OF THE TOBACCO HORNWORM, MANDUCA SEXTA: PROPERTIES AND ENDOCYTOTIC INCORPORATION INTO FOLLICLES.en_US
dc.creatorOSIR, ELLIE ONYANGO.en_US
dc.contributor.authorOSIR, ELLIE ONYANGO.en_US
dc.date.issued1986en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractManduca sexta vitellogenin is a phosphoglycolipoprotein (Mᵣ ∼ 500,000) that contains two copies of the apoproteins (apovitellogenin-I, Mᵣ 180,000 and apovitellogenin-II Mᵣ 45,000), 13 percent lipids, 3 percent carbohydrates and 0.6 percent phosphorus. The two apoproteins are immunologically distinct and apovitellogenin-II is not completely accessible to the aqueous environment in the intact molecule. The carbohydrate moiety located on apovitellogenin-I has a high mannose structure (Man₉ GlcNAc₂). Follicle membranes bind ¹²⁵I-labeled vitellogenin with high affinity and specificity (K(D) ≃ 1.3 x 10⁻⁸ M). Total binding sites were estimated at 4 x 10¹⁴ sites/g of follicle membrane protein. The binding was sensitive to pH and calcium. Competition studies showed that binding of vitellogenin was blocked by vitellin and deglycosylated vitellogenin but not by lipophorin, microvitellogenin or apovitellogenin-II. These results suggest that the uptake of vitellogenin involves binding to specific receptors on follicle membranes and the carbohydrate moiety and apovitellogenin-II are not involved in the interaction with the receptors.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectTobacco hornworm -- Eggs.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineBiochemistryen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorLaw, John H.en_US
dc.contributor.committeememberWells, Michaelen_US
dc.contributor.committeememberGrimes, Williamen_US
dc.contributor.committeememberTischler, Marcen_US
dc.contributor.committeememberBerry, Jamesen_US
dc.contributor.committeememberPrice, Ralphen_US
dc.identifier.proquest8613444en_US
dc.identifier.oclc697518980en_US
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