NONCHOLINERGIC NEUROTRANSMISSION IN THE GUINEA PIG INFERIOR MESENTERIC GANGLION: A SENSORY ROLE IN GASTROINTESTINAL PHYSIOLOGY (SYMPATHETIC, SLOW EPSP, PREVERTEBRAL, VASOPRESSIN, SUBSTANCE P).

Persistent Link:
http://hdl.handle.net/10150/188009
Title:
NONCHOLINERGIC NEUROTRANSMISSION IN THE GUINEA PIG INFERIOR MESENTERIC GANGLION: A SENSORY ROLE IN GASTROINTESTINAL PHYSIOLOGY (SYMPATHETIC, SLOW EPSP, PREVERTEBRAL, VASOPRESSIN, SUBSTANCE P).
Author:
PETERS, STEPHEN.
Issue Date:
1985
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Noncholinergic neurotransmission was studied in vitro in the guinea pig inferior mesenteric ganglion (IMG) using the technique of intracellular recording. First, the role of substance P (SP) in noncholinergic transmission was examined. Forty-four percent of IMG neurons depolarized upon superfusion of SP (1-10 x 10⁻⁷M); however, some neurons that were insensitive to SP still exhibited slow excitatory potentials (EPSPs) in response to nerve stimulation. During exposure to SP, slow EPSPs were depressed by 52% compared to paired control EPSPs. In animals treated with systemic doses of capsaicin (50-350 mg/kg), mean slow EPSP amplitude was 3.5 mV compared to 6.8 mV in untreated animals. Arginine-vasopressin (AVP) was tested for its electrophysiological effects on IMG neurons and synaptic transmission. AVP (0.5-10 x 10⁻⁷M) produced a depolarization in 67% of neurons, accompanied by an increase in membrane resistance of 44%. The depolarizations and increase in resistance were blocked by a specific V₁ receptor antagonist. During AVP-induced depolarizations, slow EPSPs were reversibly depressed in the majority of neurons by a mean of 71% relative to paired control EPSPs. The V₁ antagonist blocked slow EPSPs in only 10% of neurons tested. A group of neurons exhibiting slow EPSPs was exposed separately to both AVP and SP. Some neurons were exclusively sensitive to either SP or AVP, others were sensitive to both peptides, and still others were sensitive to neither peptide. The physiologic role of noncholinergic transmission was examined using a preparation consisting of a segment of distal colon attached to the IMG. Distension of the colon segment produced a slow depolarization resistant to cholinergic antagonists in 44% of IMG neurons. Distension-induced noncholinergic depolarizations increased in amplitude with colonic intraluminal pressure and with membrane hyperpolarization, and were accompanied by an increase in membrane input resistance of 21%. Capsaicin in vivo reduced the number of neurons exhibiting the noncholinergic mechanosensory depolarization, and in vitro capsaicin and SP desensitization reduced the amplitude of the depolarization. These results suggest that (1) both SP and AVP may be transmitters of noncholinergic potentials in the IMG, (2) some IMG neurons receive heterogeneous peptidergic innervation, and (3) noncholinergic transmission in the IMG is involved in sensory regulation of visceral autonomic function.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Sympathetic nervous system.; Gastrointestinal system.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Pharmacology and Toxicology; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Kreulen, David

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleNONCHOLINERGIC NEUROTRANSMISSION IN THE GUINEA PIG INFERIOR MESENTERIC GANGLION: A SENSORY ROLE IN GASTROINTESTINAL PHYSIOLOGY (SYMPATHETIC, SLOW EPSP, PREVERTEBRAL, VASOPRESSIN, SUBSTANCE P).en_US
dc.creatorPETERS, STEPHEN.en_US
dc.contributor.authorPETERS, STEPHEN.en_US
dc.date.issued1985en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractNoncholinergic neurotransmission was studied in vitro in the guinea pig inferior mesenteric ganglion (IMG) using the technique of intracellular recording. First, the role of substance P (SP) in noncholinergic transmission was examined. Forty-four percent of IMG neurons depolarized upon superfusion of SP (1-10 x 10⁻⁷M); however, some neurons that were insensitive to SP still exhibited slow excitatory potentials (EPSPs) in response to nerve stimulation. During exposure to SP, slow EPSPs were depressed by 52% compared to paired control EPSPs. In animals treated with systemic doses of capsaicin (50-350 mg/kg), mean slow EPSP amplitude was 3.5 mV compared to 6.8 mV in untreated animals. Arginine-vasopressin (AVP) was tested for its electrophysiological effects on IMG neurons and synaptic transmission. AVP (0.5-10 x 10⁻⁷M) produced a depolarization in 67% of neurons, accompanied by an increase in membrane resistance of 44%. The depolarizations and increase in resistance were blocked by a specific V₁ receptor antagonist. During AVP-induced depolarizations, slow EPSPs were reversibly depressed in the majority of neurons by a mean of 71% relative to paired control EPSPs. The V₁ antagonist blocked slow EPSPs in only 10% of neurons tested. A group of neurons exhibiting slow EPSPs was exposed separately to both AVP and SP. Some neurons were exclusively sensitive to either SP or AVP, others were sensitive to both peptides, and still others were sensitive to neither peptide. The physiologic role of noncholinergic transmission was examined using a preparation consisting of a segment of distal colon attached to the IMG. Distension of the colon segment produced a slow depolarization resistant to cholinergic antagonists in 44% of IMG neurons. Distension-induced noncholinergic depolarizations increased in amplitude with colonic intraluminal pressure and with membrane hyperpolarization, and were accompanied by an increase in membrane input resistance of 21%. Capsaicin in vivo reduced the number of neurons exhibiting the noncholinergic mechanosensory depolarization, and in vitro capsaicin and SP desensitization reduced the amplitude of the depolarization. These results suggest that (1) both SP and AVP may be transmitters of noncholinergic potentials in the IMG, (2) some IMG neurons receive heterogeneous peptidergic innervation, and (3) noncholinergic transmission in the IMG is involved in sensory regulation of visceral autonomic function.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectSympathetic nervous system.en_US
dc.subjectGastrointestinal system.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplinePharmacology and Toxicologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorKreulen, Daviden_US
dc.contributor.committeememberBurksen_US
dc.contributor.committeememberDrayen_US
dc.contributor.committeememberSipesen_US
dc.contributor.committeememberClaytonen_US
dc.contributor.committeememberGrueneren_US
dc.identifier.proquest8522820en_US
dc.identifier.oclc696622444en_US
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