CHARACTERIZATION OF THE GONADOTROPIN-SENSITIVE ADENYLATE CYCLASE IN THE CORPUS LUTEUM OF THE RHESUS MONKEY (MACACA MULATTA) DURING THE MENSTRUAL CYCLE.

Persistent Link:
http://hdl.handle.net/10150/187895
Title:
CHARACTERIZATION OF THE GONADOTROPIN-SENSITIVE ADENYLATE CYCLASE IN THE CORPUS LUTEUM OF THE RHESUS MONKEY (MACACA MULATTA) DURING THE MENSTRUAL CYCLE.
Author:
EYSTER, KATHLEEN MARIE.
Issue Date:
1984
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
These studies were undertaken to characterize the adenylate cyclase system of the primate (rhesus monkey) corpus luteum, and to correlate gonadotropin-sensitive adenylate cyclase activity with the functional activity of the corpus luteum at specific stages of the luteal phase of the menstrual cycle, particularly near the time of luteolysis. The conversion of [α-³²P] ATP to [³²P] cAMP was assayed in preparations of luteal tissue obtained from rhesus monkeys at midluteal phase of the menstrual cycle. Cyclic AMP production was influenced by the pH, osmolality, and ionic strength of the assay buffer, and was acutely sensitive to Mg⁺². Michaelis-Menten kinetics were seen when the ATP:Mg ratio was constant. The gonadotropins, hLH and hCG but not hFSH, stimulated cAMP production in a similar dose-dependent manner. Deglycosylated hCG blocked the stimulation of adenylate cyclase by hLH and hCG. The addition of GTP increased maximal activation of adenylate cyclase by hLH or hCG but did not alter sensitivity to the hormones. The adenylate cyclase of macaque luteal tissue did not respond to the addition of isoproterenol or epinephrine; furthermore, these catecholamines did not affect hCG-stimulation of adenylate cyclase. Forskolin and fluoride stimulated cAMP production in a dose-dependent manner. The activity of adenylate cyclase was examined in corpora lutea obtained from rhesus monkeys at specific stages in the luteal phase of the menstrual cycle (days 3-5, 6-8, 9-12, 13-15, 16-menses after the midcycle LH surge). Basal adenylate cyclase activity, activity stimulated by GMP-P(NH)P, GTP, GTP + hLH, and GTP + hCG, sensitivity of the enzyme to hLH (measured by K(act)), and peripheral progesterone levels were low in the early luteal phase (days 3-5), increased by midluteal phase (days 6-8 and 9-12), and then decreased by the late luteal phase days 13-15 and 16-menses). In contrast, there were no significant differences among the age groups tested for forskolin-stimulated activity. Thus the adenylate cyclase system of the rhesus monkey undergoes significant changes during the luteal phase which are associated with the development and regression of the corpus luteum of the menstrual cycle. Mechanisms which modulate gonadotropin and nucleotide activation of adenylate cyclase without interfering directly with the catalytic unit are implicated in the changes which accompany luteolysis.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Corpus luteum.; Luteal phase.; Ovulation.; Menstrual cycle.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Physiology; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Stouffer, Richard L.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleCHARACTERIZATION OF THE GONADOTROPIN-SENSITIVE ADENYLATE CYCLASE IN THE CORPUS LUTEUM OF THE RHESUS MONKEY (MACACA MULATTA) DURING THE MENSTRUAL CYCLE.en_US
dc.creatorEYSTER, KATHLEEN MARIE.en_US
dc.contributor.authorEYSTER, KATHLEEN MARIE.en_US
dc.date.issued1984en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThese studies were undertaken to characterize the adenylate cyclase system of the primate (rhesus monkey) corpus luteum, and to correlate gonadotropin-sensitive adenylate cyclase activity with the functional activity of the corpus luteum at specific stages of the luteal phase of the menstrual cycle, particularly near the time of luteolysis. The conversion of [α-³²P] ATP to [³²P] cAMP was assayed in preparations of luteal tissue obtained from rhesus monkeys at midluteal phase of the menstrual cycle. Cyclic AMP production was influenced by the pH, osmolality, and ionic strength of the assay buffer, and was acutely sensitive to Mg⁺². Michaelis-Menten kinetics were seen when the ATP:Mg ratio was constant. The gonadotropins, hLH and hCG but not hFSH, stimulated cAMP production in a similar dose-dependent manner. Deglycosylated hCG blocked the stimulation of adenylate cyclase by hLH and hCG. The addition of GTP increased maximal activation of adenylate cyclase by hLH or hCG but did not alter sensitivity to the hormones. The adenylate cyclase of macaque luteal tissue did not respond to the addition of isoproterenol or epinephrine; furthermore, these catecholamines did not affect hCG-stimulation of adenylate cyclase. Forskolin and fluoride stimulated cAMP production in a dose-dependent manner. The activity of adenylate cyclase was examined in corpora lutea obtained from rhesus monkeys at specific stages in the luteal phase of the menstrual cycle (days 3-5, 6-8, 9-12, 13-15, 16-menses after the midcycle LH surge). Basal adenylate cyclase activity, activity stimulated by GMP-P(NH)P, GTP, GTP + hLH, and GTP + hCG, sensitivity of the enzyme to hLH (measured by K(act)), and peripheral progesterone levels were low in the early luteal phase (days 3-5), increased by midluteal phase (days 6-8 and 9-12), and then decreased by the late luteal phase days 13-15 and 16-menses). In contrast, there were no significant differences among the age groups tested for forskolin-stimulated activity. Thus the adenylate cyclase system of the rhesus monkey undergoes significant changes during the luteal phase which are associated with the development and regression of the corpus luteum of the menstrual cycle. Mechanisms which modulate gonadotropin and nucleotide activation of adenylate cyclase without interfering directly with the catalytic unit are implicated in the changes which accompany luteolysis.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectCorpus luteum.en_US
dc.subjectLuteal phase.en_US
dc.subjectOvulation.en_US
dc.subjectMenstrual cycle.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplinePhysiologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorStouffer, Richard L.en_US
dc.identifier.proquest8506958en_US
dc.identifier.oclc693591954en_US
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