LOCALIZATION AND COMPLETE NUCLEOTIDE SEQUENCE OF THE BOVINE PAPILLOMAVIRUS TYPE-2 LATE REGION. POLYPEPTIDE COMPOSITION OF BPV-2.

Persistent Link:
http://hdl.handle.net/10150/187657
Title:
LOCALIZATION AND COMPLETE NUCLEOTIDE SEQUENCE OF THE BOVINE PAPILLOMAVIRUS TYPE-2 LATE REGION. POLYPEPTIDE COMPOSITION OF BPV-2.
Author:
POTTER, HAROLD LEE, JR.
Issue Date:
1984
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
This investigation was designed to partially characterize the genome of bovine papillomavirus type-2 (BPV-2). A closely related virus, bovine papillomavirus type-1 (BPV-1), has been extensively studied. Additionally, many members of the human papillomaviruses (HPV) are being investigated in great detail. However, only a few molecular biology studies of BPV-2 have been reported. Therefore, it seemed appropriate to initiate an investigation whose results would provide a good basis for continued studies of BPV-2. A detailed physical map of BPV-2 DNA was prepared by restriction endonuclease digestion of BPV-2 DNA. A comparison between the genomes of BPV-2 and BPV-1 in regard to location and number of cleavage sites of seven enzymes was performed. This comparison revealed similarities between the two genomes and indicated the probable location of the BPV-2 late region by direct extrapolation from the BPV-1 genomic map. This area was completely sequenced by the chemical method and the results confirmed it to indeed be the BPV-2 late region. Comparative analysis between the BPV-2 late region and the BPV-1 late region demonstrated identical genetic organization to exist along with a high degree of nucleotide sequence conservation. Also, the polypeptide composition of BPV-2 was determined and similarities with these and BPV-1 polypeptides were noted. Lastly, a discussion of the BPV-2 polypeptides as potential products of the BPV-2 late region is presented.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Nucleotide sequence.; Papovaviruses.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Microbiology and Immunology; Graduate College
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleLOCALIZATION AND COMPLETE NUCLEOTIDE SEQUENCE OF THE BOVINE PAPILLOMAVIRUS TYPE-2 LATE REGION. POLYPEPTIDE COMPOSITION OF BPV-2.en_US
dc.creatorPOTTER, HAROLD LEE, JR.en_US
dc.contributor.authorPOTTER, HAROLD LEE, JR.en_US
dc.date.issued1984en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThis investigation was designed to partially characterize the genome of bovine papillomavirus type-2 (BPV-2). A closely related virus, bovine papillomavirus type-1 (BPV-1), has been extensively studied. Additionally, many members of the human papillomaviruses (HPV) are being investigated in great detail. However, only a few molecular biology studies of BPV-2 have been reported. Therefore, it seemed appropriate to initiate an investigation whose results would provide a good basis for continued studies of BPV-2. A detailed physical map of BPV-2 DNA was prepared by restriction endonuclease digestion of BPV-2 DNA. A comparison between the genomes of BPV-2 and BPV-1 in regard to location and number of cleavage sites of seven enzymes was performed. This comparison revealed similarities between the two genomes and indicated the probable location of the BPV-2 late region by direct extrapolation from the BPV-1 genomic map. This area was completely sequenced by the chemical method and the results confirmed it to indeed be the BPV-2 late region. Comparative analysis between the BPV-2 late region and the BPV-1 late region demonstrated identical genetic organization to exist along with a high degree of nucleotide sequence conservation. Also, the polypeptide composition of BPV-2 was determined and similarities with these and BPV-1 polypeptides were noted. Lastly, a discussion of the BPV-2 polypeptides as potential products of the BPV-2 late region is presented.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectNucleotide sequence.en_US
dc.subjectPapovaviruses.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineMicrobiology and Immunologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.committeememberSpizizen, Johnen_US
dc.contributor.committeememberKibler, Ruthen_US
dc.contributor.committeememberHewlett, Martinzezen_US
dc.contributor.committeememberHall, Jenniferen_US
dc.identifier.proquest8412674en_US
dc.identifier.oclc690929119en_US
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.