Studies of 3-hydroxy-3-methylglutaryl coenzyme A reductase expression during Zea mays seed development.

Persistent Link:
http://hdl.handle.net/10150/187096
Title:
Studies of 3-hydroxy-3-methylglutaryl coenzyme A reductase expression during Zea mays seed development.
Author:
Moore, Kathryn Blackford.
Issue Date:
1995
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The first committed step in isoprenoid biosynthesis is the conversion of 3-hydroxy-3-methylglutaryl coenzyme A to mevalonate, which is catalyzed by the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR). To begin to understand the role of isoprenoid synthesis in maize, the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase was examined. This study shows that the specific activity of the rate-limiting enzyme, HMGR, is regulated during Zea mays development. High levels of activity were correlated with stages of rapid, mitotic divisions. During seed development, HMGR specific activity is differentially regulated in the embryo versus the endosperm. During the transition from mitotic division to cell expansion in the seed, HMGR activity may represent a unique biochemical marker. The HMGR activity does not appear to be regulated at the levels of mRNA or protein accumulation. HMGR specific activity is inversely correlated with endogenous abscisic acid levels in Zea mays endosperm. HMGR activity was increased in the endosperm of three vivipary mutants, vp2, vp5, and vp7, which are defective in abscisic acid biosynthesis by the zygote. Examination of vp8 mutants revealed that high HMGR specific activity also was correlated with reductions in endogenous levels of maternally-derived abscisic acid. Abscisic acid appears to regulate HMGR throughout late embryogenesis and maturation. In addition, the HMGR specific activity was increased in the vp1 mutant, which is defective in an abscisic acid response element. There was no correlation between HMGR protein levels and specific activity patterns in these mutants. Exogenous abscisic acid inhibits HMGR specific activity in maize roots. Application of abscisic acid results in decreased HMGR transcript levels, and the inhibition of activity is protein kinase-dependent. Although abscisic acid is a negative regulator of HMGR, there appear to be other signals which set the overall pattern and level of HMGR activity and override the influence of the phytohormone. Examination of HMGR specific activity in vivipary embryos, demonstrated that signals which set the developmental stage of the tissue, also dictate the patterns of enzyme expression. What signals determine the developmental expression of HMGR remains to be determined.
Type:
text; Dissertation-Reproduction (electronic)
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Molecular and Cellular Biology; Graduate College
Degree Grantor:
University of Arizona
Committee Chair:
Oishi, Karen

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleStudies of 3-hydroxy-3-methylglutaryl coenzyme A reductase expression during Zea mays seed development.en_US
dc.creatorMoore, Kathryn Blackford.en_US
dc.contributor.authorMoore, Kathryn Blackford.en_US
dc.date.issued1995en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe first committed step in isoprenoid biosynthesis is the conversion of 3-hydroxy-3-methylglutaryl coenzyme A to mevalonate, which is catalyzed by the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR). To begin to understand the role of isoprenoid synthesis in maize, the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase was examined. This study shows that the specific activity of the rate-limiting enzyme, HMGR, is regulated during Zea mays development. High levels of activity were correlated with stages of rapid, mitotic divisions. During seed development, HMGR specific activity is differentially regulated in the embryo versus the endosperm. During the transition from mitotic division to cell expansion in the seed, HMGR activity may represent a unique biochemical marker. The HMGR activity does not appear to be regulated at the levels of mRNA or protein accumulation. HMGR specific activity is inversely correlated with endogenous abscisic acid levels in Zea mays endosperm. HMGR activity was increased in the endosperm of three vivipary mutants, vp2, vp5, and vp7, which are defective in abscisic acid biosynthesis by the zygote. Examination of vp8 mutants revealed that high HMGR specific activity also was correlated with reductions in endogenous levels of maternally-derived abscisic acid. Abscisic acid appears to regulate HMGR throughout late embryogenesis and maturation. In addition, the HMGR specific activity was increased in the vp1 mutant, which is defective in an abscisic acid response element. There was no correlation between HMGR protein levels and specific activity patterns in these mutants. Exogenous abscisic acid inhibits HMGR specific activity in maize roots. Application of abscisic acid results in decreased HMGR transcript levels, and the inhibition of activity is protein kinase-dependent. Although abscisic acid is a negative regulator of HMGR, there appear to be other signals which set the overall pattern and level of HMGR activity and override the influence of the phytohormone. Examination of HMGR specific activity in vivipary embryos, demonstrated that signals which set the developmental stage of the tissue, also dictate the patterns of enzyme expression. What signals determine the developmental expression of HMGR remains to be determined.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineMolecular and Cellular Biologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.chairOishi, Karenen_US
dc.contributor.committeememberWard, Samuelen_US
dc.contributor.committeememberVierling, Elizabethen_US
dc.contributor.committeememberBohnert, Hansen_US
dc.contributor.committeememberHawes, Marthaen_US
dc.identifier.proquest9531116en_US
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