Photoinduced fusion of liposomes.

http://hdl.handle.net/10150/187055
Title:
Photoinduced fusion of liposomes.
Author:
Bennett, Doyle Edward.
Issue Date:
1995
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The photopolymerization of two-component large unilamellar liposomes (LUV) composed of 3/1 dioleoylphosphatidylethanolamine (DOPE) and either 1,2-bis- (10-(2',4'-hexadienoyloxy)decanoyl) -sn-glycero-3-phosphatidylcholine (bis-SorbPC) or 1-palmitoyl-2- (10-(2$\sp\prime,4\sp\prime$-hexadienoyloxy) decanoyl) -sn-glycero-3-phosphocholine (mono-SorbPC) facilitated liposome fusion. Fusion was characterized by fluorescent assays for lipid mixing, aqueous contents mixing, and aqueous contents leakage. The rate and extent of the photoinduced fusion was dependent on the extent of polymerization, temperature, and the fusion initiation conditions, including the pH and the presence of Mg²⁺ ions. Examination of the temperature dependence of fusion for unpolymerized and polymerized liposomes showed that an enhancement of the rate of fusion occurred in the temperature range, Δ T(I), where inverted phase lipid intermediates are observed. Photopolymerization causes lateral separation of the liposome components and thereby results in the isothermal induction of fusion by lowering the threshold temperature for formation of fusion intermediates.
Type:
text; Dissertation-Reproduction (electronic)
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Degree Grantor:
University of Arizona
Committee Chair:
O'Brien, David F.

DC FieldValue Language
dc.language.isoenen_US
dc.titlePhotoinduced fusion of liposomes.en_US
dc.creatorBennett, Doyle Edward.en_US
dc.contributor.authorBennett, Doyle Edward.en_US
dc.date.issued1995en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe photopolymerization of two-component large unilamellar liposomes (LUV) composed of 3/1 dioleoylphosphatidylethanolamine (DOPE) and either 1,2-bis- (10-(2',4'-hexadienoyloxy)decanoyl) -sn-glycero-3-phosphatidylcholine (bis-SorbPC) or 1-palmitoyl-2- (10-(2$\sp\prime,4\sp\prime$-hexadienoyloxy) decanoyl) -sn-glycero-3-phosphocholine (mono-SorbPC) facilitated liposome fusion. Fusion was characterized by fluorescent assays for lipid mixing, aqueous contents mixing, and aqueous contents leakage. The rate and extent of the photoinduced fusion was dependent on the extent of polymerization, temperature, and the fusion initiation conditions, including the pH and the presence of Mg²⁺ ions. Examination of the temperature dependence of fusion for unpolymerized and polymerized liposomes showed that an enhancement of the rate of fusion occurred in the temperature range, Δ T(I), where inverted phase lipid intermediates are observed. Photopolymerization causes lateral separation of the liposome components and thereby results in the isothermal induction of fusion by lowering the threshold temperature for formation of fusion intermediates.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineChemistryen_US