Molecular biology and evolution of the Rubisco small subunit multi-gene family in Mesembryanthemum crystallinum.

Persistent Link:
http://hdl.handle.net/10150/185987
Title:
Molecular biology and evolution of the Rubisco small subunit multi-gene family in Mesembryanthemum crystallinum.
Author:
De Rocher, Ernest Jay.
Issue Date:
1992
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The genes that comprise the multi-gene family encoding the small subunit of the CO₂ fixing enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in the facultative Crassulacean Acid Metabolism (CAM) plant Mesembryanthemum crystallinum were isolated and nucleotide sequences were determined. The gene family is comprised of six genes of which four are expressed (rbcS-1, rbcS-2, rbcS-3 and rbcS-4) based on the isolation of corresponding cDNAs and northern analysis. Sequence analysis of the genes reveals influences of gene duplication, gene conversion and selection pressure in the evolution of the gene family. Five of the genes are located in a tandem array within 20 kbp. Two are identical throughout the coding regions and intron sequences. The promoter regions of five of the genes lack regulatory sequence elements conserved in other dicot species. Gene specific probes were used to determine the expression properties of the rbcS-1, rbcS-2, rbcS-3 and rbcS-4 genes during environmental stress, development and the diurnal cycle. The four genes are differentially expressed and are co-regulated in response to stress and during development. Steady state rbcS mRNA amounts are down regulated by NaCl and cold stress. Comparison of changes in transcriptional activities relative to steady state mRNA amounts revealed that NaCl causes increased rbcS RNA turnover. RbcS expression is transcriptionally down regulated coincident with the developmental enablement of CAM induction by stress. The regulation of rbcS expression by changes in RNA turnover is itself developmentally regulated. The rbcS genes of M. crystallinum are transcriptionally regulated during development and are regulated at the level of RNA turnover by stress.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Dissertations, Academic.; Molecular biology.; Ice plant.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Molecular and Cellular Biology; Graduate College
Degree Grantor:
University of Arizona
Committee Chair:
Bohnert, Hans J.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleMolecular biology and evolution of the Rubisco small subunit multi-gene family in Mesembryanthemum crystallinum.en_US
dc.creatorDe Rocher, Ernest Jay.en_US
dc.contributor.authorDe Rocher, Ernest Jay.en_US
dc.date.issued1992en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe genes that comprise the multi-gene family encoding the small subunit of the CO₂ fixing enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in the facultative Crassulacean Acid Metabolism (CAM) plant Mesembryanthemum crystallinum were isolated and nucleotide sequences were determined. The gene family is comprised of six genes of which four are expressed (rbcS-1, rbcS-2, rbcS-3 and rbcS-4) based on the isolation of corresponding cDNAs and northern analysis. Sequence analysis of the genes reveals influences of gene duplication, gene conversion and selection pressure in the evolution of the gene family. Five of the genes are located in a tandem array within 20 kbp. Two are identical throughout the coding regions and intron sequences. The promoter regions of five of the genes lack regulatory sequence elements conserved in other dicot species. Gene specific probes were used to determine the expression properties of the rbcS-1, rbcS-2, rbcS-3 and rbcS-4 genes during environmental stress, development and the diurnal cycle. The four genes are differentially expressed and are co-regulated in response to stress and during development. Steady state rbcS mRNA amounts are down regulated by NaCl and cold stress. Comparison of changes in transcriptional activities relative to steady state mRNA amounts revealed that NaCl causes increased rbcS RNA turnover. RbcS expression is transcriptionally down regulated coincident with the developmental enablement of CAM induction by stress. The regulation of rbcS expression by changes in RNA turnover is itself developmentally regulated. The rbcS genes of M. crystallinum are transcriptionally regulated during development and are regulated at the level of RNA turnover by stress.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectDissertations, Academic.en_US
dc.subjectMolecular biology.en_US
dc.subjectIce plant.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineMolecular and Cellular Biologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.chairBohnert, Hans J.-
dc.contributor.committeememberVierling, Elizabethen_US
dc.contributor.committeememberOishi, Karenen_US
dc.contributor.committeememberRay, Dennisen_US
dc.contributor.committeememberBurd, Gailen_US
dc.identifier.proquest9307652en_US
dc.identifier.oclc713865211en_US
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