Persistent Link:
http://hdl.handle.net/10150/185786
Title:
DNA replication in Drosophila embryos: Proteins at the fork.
Author:
Peck, Vickie Marie.
Issue Date:
1992
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Drosophila embryos provide a rich source of replicative enzymes. Also, the duration of 2 hour embryo DNA synthesis phase of the cell cycle is approximately 6-fold shorter than the more regulated 9 hour embryo S phase. Thus, Drosophila embryos are a good system in which to explore the mechanisms and regulation of DNA replication. Early stage, 2 hour embryos contain at least two distinct DNA polymerases, DNA polymerase α and δ, as determined by associated enzymatic activities (DNA primase and 3'-5' exonuclease), inhibitor studies, immunologic reactivity, and processivity measurements. The observation that a δ-type enzyme with an inherent 3'-5' exonuclease activity is present in Drosophila embryos is a novel observation, and may have important implications for maintaining the fidelity of embryonic DNA synthesis. Both 2 hour and 9 hour embryos contained similar replicative activities. The enzymes which copurified with 2 hour and 9 hour DNA polymerases include a DNA primase activity with DNA polymerase α; and a 3'-5' exonuclease, 5'-3' exonuclease, and DNA ligase activities with DNA polymerase δ. The association of these activities suggests that DNA polymerase α-associated enzymes may initiate Okazaki fragments, which would then be elongated and ligated by the DNA polymerase δ-associated group.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Dissertations, Academic.; DNA replication.; Biochemistry.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Microbiology and Immunology; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Cress, Anne E.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleDNA replication in Drosophila embryos: Proteins at the fork.en_US
dc.creatorPeck, Vickie Marie.en_US
dc.contributor.authorPeck, Vickie Marie.en_US
dc.date.issued1992en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractDrosophila embryos provide a rich source of replicative enzymes. Also, the duration of 2 hour embryo DNA synthesis phase of the cell cycle is approximately 6-fold shorter than the more regulated 9 hour embryo S phase. Thus, Drosophila embryos are a good system in which to explore the mechanisms and regulation of DNA replication. Early stage, 2 hour embryos contain at least two distinct DNA polymerases, DNA polymerase α and δ, as determined by associated enzymatic activities (DNA primase and 3'-5' exonuclease), inhibitor studies, immunologic reactivity, and processivity measurements. The observation that a δ-type enzyme with an inherent 3'-5' exonuclease activity is present in Drosophila embryos is a novel observation, and may have important implications for maintaining the fidelity of embryonic DNA synthesis. Both 2 hour and 9 hour embryos contained similar replicative activities. The enzymes which copurified with 2 hour and 9 hour DNA polymerases include a DNA primase activity with DNA polymerase α; and a 3'-5' exonuclease, 5'-3' exonuclease, and DNA ligase activities with DNA polymerase δ. The association of these activities suggests that DNA polymerase α-associated enzymes may initiate Okazaki fragments, which would then be elongated and ligated by the DNA polymerase δ-associated group.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectDissertations, Academic.en_US
dc.subjectDNA replication.en_US
dc.subjectBiochemistry.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineMicrobiology and Immunologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorCress, Anne E.en_US
dc.contributor.committeememberHendrix, Mary J.C.en_US
dc.contributor.committeememberBernstein, Harrisen_US
dc.contributor.committeememberDieckmann, Carolen_US
dc.identifier.proquest9223555en_US
dc.identifier.oclc712217542en_US
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