Species-specific factors affecting the population dynamics of P transposable elements in Drosophila melanogaster and Drosophila simulans.

Persistent Link:
http://hdl.handle.net/10150/185657
Title:
Species-specific factors affecting the population dynamics of P transposable elements in Drosophila melanogaster and Drosophila simulans.
Author:
Kimura, Kiyoshi.
Issue Date:
1991
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
In order to compare the population dynamics of P elements in the sibling species D. melanogaster and D. simulans, three sets of studies were performed. In a series of comparative mixed population experiments, P-M mixed populations of D. melanogaster evolved into P or Q populations, depending on the strength of the P strains used as the original parental strains. However, no mixed populations of D. simulans evolved into P populations; even when the strongest P strains of D. simulans were used, all mixed populations evolved into Q or M' populations. Using an excision assay system, excision frequencies in a variety of strains of D. melanogaster and D. simulans were measured. On average, excision frequencies of P elements from plasmids were significantly lower in D. simulans than in D. melanogaster. In addition, there were no statistically significant correlations between GD sterility and excision frequencies. Using single neomycin -marked P elements, transposition frequencies of P elements were compared in D. melanogaster and D. simulans. As with excision frequencies, transposition frequencies were lower in D. simulans than in D. melanogaster. In addition, the position of the original insertion site had a strong effect on transposition frequency. All of these three experiments showed significantly lower average activity of P elements in D. simulans than in D. melanogaster. This consistency suggests that the three sets of results were influenced either directly and/or indirectly by common factors, such as host-encoded factors. Therefore, differences in the activity of P elements in these two species may be influenced by different host-encoded factors. The absence of P elements in natural populations of D. simulans may also be explained by the presence of different host factors.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Dissertations, Academic; Genetics; Population biology
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Ecology and Evolutionary Biology; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Kidwell, Margaret G.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleSpecies-specific factors affecting the population dynamics of P transposable elements in Drosophila melanogaster and Drosophila simulans.en_US
dc.creatorKimura, Kiyoshi.en_US
dc.contributor.authorKimura, Kiyoshi.en_US
dc.date.issued1991en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractIn order to compare the population dynamics of P elements in the sibling species D. melanogaster and D. simulans, three sets of studies were performed. In a series of comparative mixed population experiments, P-M mixed populations of D. melanogaster evolved into P or Q populations, depending on the strength of the P strains used as the original parental strains. However, no mixed populations of D. simulans evolved into P populations; even when the strongest P strains of D. simulans were used, all mixed populations evolved into Q or M' populations. Using an excision assay system, excision frequencies in a variety of strains of D. melanogaster and D. simulans were measured. On average, excision frequencies of P elements from plasmids were significantly lower in D. simulans than in D. melanogaster. In addition, there were no statistically significant correlations between GD sterility and excision frequencies. Using single neomycin -marked P elements, transposition frequencies of P elements were compared in D. melanogaster and D. simulans. As with excision frequencies, transposition frequencies were lower in D. simulans than in D. melanogaster. In addition, the position of the original insertion site had a strong effect on transposition frequency. All of these three experiments showed significantly lower average activity of P elements in D. simulans than in D. melanogaster. This consistency suggests that the three sets of results were influenced either directly and/or indirectly by common factors, such as host-encoded factors. Therefore, differences in the activity of P elements in these two species may be influenced by different host-encoded factors. The absence of P elements in natural populations of D. simulans may also be explained by the presence of different host factors.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectDissertations, Academicen_US
dc.subjectGeneticsen_US
dc.subjectPopulation biologyen_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineEcology and Evolutionary Biologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorKidwell, Margaret G.en_US
dc.contributor.committeememberHeed, William B.en_US
dc.contributor.committeememberWalsh, J. Bruceen_US
dc.contributor.committeememberBrower, Dannyen_US
dc.contributor.committeememberOishi, Karen K.en_US
dc.identifier.proquest9208056en_US
dc.identifier.oclc711880767en_US
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