Characterization and differentiation of a soluble lipopolysaccharide type antigen from Treponema hyodysenteriae.

Persistent Link:
http://hdl.handle.net/10150/185111
Title:
Characterization and differentiation of a soluble lipopolysaccharide type antigen from Treponema hyodysenteriae.
Author:
Halter, Mitchell Roy.
Issue Date:
1990
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Whole cells of T. hyodysenteriae serotypes 1-7, avirulent T. hyodysenteriae serotypes 1 and 2, and 5 strains of T. innocens were chemically extracted to selectively remove a lipopolysaccharide-like substance (LPSLS). The different LPSLS were analyzed electrophoretically, immunologically, and chemically. SDS-PAGE demonstrated migratory differences that were unique for individual serotypes/strains. Additional differences were observed during attenuation which resulted in reduced mobility of upper molecular weight components. Western blotting with hyper-immunized rabbit serum (HRS) against serotype 1, 2, and 6 whole cell bacterins produced homologous and heterologous reactions with serotype specific LPSLS. Rabbit antisera to serotypes 3, 4, 5, and 7 whole cell bacterins produced only homologous reaction to the LPSLS. Convalescent-phase swine sera (CSS) against serotype 1 disease showed only homologous reaction to the LPSLS. Convalescent-phase swine sera against serotype 2 produced both homologous and heterologous reaction to the LPSLS. Antigenicity was recognized by HRS and CSS to the hydrophobic and hydrophilic components of acid hydrolyzed LPSLS. Thin layer chromatography (TLC) of the hydrophobic portion demonstrated the presence of a spot with an Rf of 0.18 that correlates with nonpathogenicity. Gas chromatography (GC) analysis of the carbohydrate content of the LPSLS revealed the presence of glucose, galactose, N-acetyl neuraminic acid, N-acetyl glucosamine, and N-acetyl galactosamine. 2-keto-3-deoxyoctulonate (KDO) and L-glycero-D-manno-heptose were absent. The LPSLS from T. hyodysenteriae was found to have low anticomplement activity in comparison with LPS. The LPSLS from T. innocens had one half the activity of LPS. T. hyodysenteriae LPSLS demonstrated comparable activity to LPS in inducing Ia expression by macrophages. The LPSLS from T. innocens was unable to stimulate expression of a molecules on macrophages.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Biology
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Microbiology and Immunology; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Joens, Lynn A.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleCharacterization and differentiation of a soluble lipopolysaccharide type antigen from Treponema hyodysenteriae.en_US
dc.creatorHalter, Mitchell Roy.en_US
dc.contributor.authorHalter, Mitchell Roy.en_US
dc.date.issued1990en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractWhole cells of T. hyodysenteriae serotypes 1-7, avirulent T. hyodysenteriae serotypes 1 and 2, and 5 strains of T. innocens were chemically extracted to selectively remove a lipopolysaccharide-like substance (LPSLS). The different LPSLS were analyzed electrophoretically, immunologically, and chemically. SDS-PAGE demonstrated migratory differences that were unique for individual serotypes/strains. Additional differences were observed during attenuation which resulted in reduced mobility of upper molecular weight components. Western blotting with hyper-immunized rabbit serum (HRS) against serotype 1, 2, and 6 whole cell bacterins produced homologous and heterologous reactions with serotype specific LPSLS. Rabbit antisera to serotypes 3, 4, 5, and 7 whole cell bacterins produced only homologous reaction to the LPSLS. Convalescent-phase swine sera (CSS) against serotype 1 disease showed only homologous reaction to the LPSLS. Convalescent-phase swine sera against serotype 2 produced both homologous and heterologous reaction to the LPSLS. Antigenicity was recognized by HRS and CSS to the hydrophobic and hydrophilic components of acid hydrolyzed LPSLS. Thin layer chromatography (TLC) of the hydrophobic portion demonstrated the presence of a spot with an Rf of 0.18 that correlates with nonpathogenicity. Gas chromatography (GC) analysis of the carbohydrate content of the LPSLS revealed the presence of glucose, galactose, N-acetyl neuraminic acid, N-acetyl glucosamine, and N-acetyl galactosamine. 2-keto-3-deoxyoctulonate (KDO) and L-glycero-D-manno-heptose were absent. The LPSLS from T. hyodysenteriae was found to have low anticomplement activity in comparison with LPS. The LPSLS from T. innocens had one half the activity of LPS. T. hyodysenteriae LPSLS demonstrated comparable activity to LPS in inducing Ia expression by macrophages. The LPSLS from T. innocens was unable to stimulate expression of a molecules on macrophages.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectBiologyen_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineMicrobiology and Immunologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorJoens, Lynn A.en_US
dc.contributor.committeememberFriedman, Richarden_US
dc.contributor.committeememberRyan, Kennethen_US
dc.contributor.committeememberSterling, Charlesen_US
dc.contributor.committeememberSammons, Daviden_US
dc.identifier.proquest9100041en_US
dc.identifier.oclc708399329en_US
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