Fluorescence enhancement and angular scattering by selected cylindrical particles.

Persistent Link:
http://hdl.handle.net/10150/185041
Title:
Fluorescence enhancement and angular scattering by selected cylindrical particles.
Author:
Abromson, David, 1961-
Issue Date:
1990
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
An examination of light scattering and emissions from a fluorescent dye confined within small hollow core quartz fibers has revealed several interesting phenomena. First, backscattered fluorescent emissions increased as the fiber's inner diameter decreased below 23 microns. Second, interference between the inner and outer fiber walls created angular scattering intensity oscillations in the elastic scattered light and fluorescent emissions. The interference induced oscillations produced large intensity changes in less than a degree. Third, fiber immersion in an index-matched fluid removed the interference oscillations, and fourth, the fiber immersion also decreased the backscattered fluorescent emissions for the same fiber. These research results can be used to optimize the design of fluorescence measuring devices used in capillary zone electrophoresis (CZE). A CZE device measures the fluorescence spectrum from biological material within small hollow core fibers. The small amount of fluorescent material makes it desirable to find an optimum viewing angle to maximize signal and avoid spurious results from selective wavelength enhancement and intensity variations in angular scattering caused by the fiber.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Physics
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Physics; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Bickel, W.S.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleFluorescence enhancement and angular scattering by selected cylindrical particles.en_US
dc.creatorAbromson, David, 1961-en_US
dc.contributor.authorAbromson, David, 1961-en_US
dc.date.issued1990en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractAn examination of light scattering and emissions from a fluorescent dye confined within small hollow core quartz fibers has revealed several interesting phenomena. First, backscattered fluorescent emissions increased as the fiber's inner diameter decreased below 23 microns. Second, interference between the inner and outer fiber walls created angular scattering intensity oscillations in the elastic scattered light and fluorescent emissions. The interference induced oscillations produced large intensity changes in less than a degree. Third, fiber immersion in an index-matched fluid removed the interference oscillations, and fourth, the fiber immersion also decreased the backscattered fluorescent emissions for the same fiber. These research results can be used to optimize the design of fluorescence measuring devices used in capillary zone electrophoresis (CZE). A CZE device measures the fluorescence spectrum from biological material within small hollow core fibers. The small amount of fluorescent material makes it desirable to find an optimum viewing angle to maximize signal and avoid spurious results from selective wavelength enhancement and intensity variations in angular scattering caused by the fiber.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectPhysicsen_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplinePhysicsen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorBickel, W.S.en_US
dc.contributor.committeememberHuffman, D.en_US
dc.contributor.committeememberLeavitt, J.en_US
dc.contributor.committeememberStoner, J.en_US
dc.contributor.committeememberJust, K.en_US
dc.identifier.proquest9025061en_US
dc.identifier.oclc708248073en_US
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