PRESENCE OF INOSINE MONOPHOSPHATE DURING CELL MEDIATED IMMUNITY IN GUINEA PIGS (IMP).

Persistent Link:
http://hdl.handle.net/10150/184714
Title:
PRESENCE OF INOSINE MONOPHOSPHATE DURING CELL MEDIATED IMMUNITY IN GUINEA PIGS (IMP).
Author:
VALENTINE, MARY ANN.
Issue Date:
1982
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The presence of the purine nucleotide inosine monophosphate (IMP) was studied in direct relationship to the development and expression of cell mediated immunity in guinea pigs using DNCB or Histoplasma capsulatum as sensitizing antigens. The IMP content of T-cell enriched lymphocytic lysates was measured by isocratic high pressure liquid chromatography (HPLC). Intracellular IMP levels of cells from homologously skin tested sensitized animals were significantly increased one day after skin testing when compared to the concentrations found in these cells during the period following sensitization. Concurrent with these observations were the findings that the absolute lymphocyte counts and histoplasmin stimulated in vitro blastogenic responses increased following sensitization while the PHA-induced proliferative response decreased slightly. One day after skin testing, when IMP levels had increased, there was a slight decrease in lymphocyte numbers and a marked decrease in the PHA response. Cells collected at this time and cultured in vitro with histoplasmin responded with increased levels of protein production and increased IMP levels. These data suggest (1) the proliferative response of cells from sensitized animals appears to be associated with lower levels of intracellular IMP, and (2) sensitized cells stimulated in vivo with antigen appear to have characteristically higher IMP concentrations.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Purine nucleotides.; Lymphocytes.; Phytohemagglutinins.; Cellular immunity.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Microbiology; Graduate College
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titlePRESENCE OF INOSINE MONOPHOSPHATE DURING CELL MEDIATED IMMUNITY IN GUINEA PIGS (IMP).en_US
dc.creatorVALENTINE, MARY ANN.en_US
dc.contributor.authorVALENTINE, MARY ANN.en_US
dc.date.issued1982en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe presence of the purine nucleotide inosine monophosphate (IMP) was studied in direct relationship to the development and expression of cell mediated immunity in guinea pigs using DNCB or Histoplasma capsulatum as sensitizing antigens. The IMP content of T-cell enriched lymphocytic lysates was measured by isocratic high pressure liquid chromatography (HPLC). Intracellular IMP levels of cells from homologously skin tested sensitized animals were significantly increased one day after skin testing when compared to the concentrations found in these cells during the period following sensitization. Concurrent with these observations were the findings that the absolute lymphocyte counts and histoplasmin stimulated in vitro blastogenic responses increased following sensitization while the PHA-induced proliferative response decreased slightly. One day after skin testing, when IMP levels had increased, there was a slight decrease in lymphocyte numbers and a marked decrease in the PHA response. Cells collected at this time and cultured in vitro with histoplasmin responded with increased levels of protein production and increased IMP levels. These data suggest (1) the proliferative response of cells from sensitized animals appears to be associated with lower levels of intracellular IMP, and (2) sensitized cells stimulated in vivo with antigen appear to have characteristically higher IMP concentrations.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectPurine nucleotides.en_US
dc.subjectLymphocytes.en_US
dc.subjectPhytohemagglutinins.en_US
dc.subjectCellular immunity.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineMicrobiologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.identifier.proquest8227374en_US
dc.identifier.oclc682964816en_US
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