The modification of nutritional and functional properties of chickpea (Cicer arietinum) by germination.

Persistent Link:
http://hdl.handle.net/10150/184363
Title:
The modification of nutritional and functional properties of chickpea (Cicer arietinum) by germination.
Author:
Fernandez, Maria Luz.
Issue Date:
1988
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Chickpea (Cicer Arietinum) was germinated for different lengths of time to determine the influence of germination on the functional and nutritional properties of this legume. Chemical analysis of the flours showed a very significant increase in vitamin C and in lysine during germination. Vitamin C values ranged from 1.2 to 15.6 mg/100 g and lysine from 10.5 to 13.5 g/100g of protein for the intact and the 48 hr-germinated chickpea, respectively. Starch content decreased 15.5% and soluble sugars increased 20% after only 24 hr of germination. Germination decreased trypsin inhibitor activity by 28%. Chickpea and 24 hr germinated chickpea were used as ingredients in the preparation of several products. Germination increased acceptability in some of these products by modifying their rheological and sensory properties. Seed germination enhanced significantly the nutritional quality of chickpea protein. Protein efficiency ratio associated with the germinated chickpea diets compared favorably to that obtained with the casein diet. Protein digestibility decreased as germination time increased. Essential amino acid availability did not change after 24 hr of germination, but small decreases were observed after 48 hr. Protein and starch were studied separately to determine their influence on the observed modifications. No significant changes were found in the concentration of proteins in germinated chickpea even after 72 hr of germination as indicated by densitometry scans of SDS-PAGE patterns. Starch was isolated from intact and germinated chickpeas and characterized by several of its physicochemical properties and its susceptibility to alpha-amylase hydrolysis. Germination increased substantially starch digestibility and modified some of the physico-chemical properties of starch. Scanning electron microscopy (SEM) showed no apparent differences between starches except for a tendency of the germinated chickpea starch to clump. These results suggest that changes in texture, consistency and other physical parameters observed on the germinated chickpea-based products may be attributed mostly to starch.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Chickpea -- Nutrition.; Germination.; Food -- Protein content.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Nutritional Sciences; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Berry, James W.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleThe modification of nutritional and functional properties of chickpea (Cicer arietinum) by germination.en_US
dc.creatorFernandez, Maria Luz.en_US
dc.contributor.authorFernandez, Maria Luz.en_US
dc.date.issued1988en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractChickpea (Cicer Arietinum) was germinated for different lengths of time to determine the influence of germination on the functional and nutritional properties of this legume. Chemical analysis of the flours showed a very significant increase in vitamin C and in lysine during germination. Vitamin C values ranged from 1.2 to 15.6 mg/100 g and lysine from 10.5 to 13.5 g/100g of protein for the intact and the 48 hr-germinated chickpea, respectively. Starch content decreased 15.5% and soluble sugars increased 20% after only 24 hr of germination. Germination decreased trypsin inhibitor activity by 28%. Chickpea and 24 hr germinated chickpea were used as ingredients in the preparation of several products. Germination increased acceptability in some of these products by modifying their rheological and sensory properties. Seed germination enhanced significantly the nutritional quality of chickpea protein. Protein efficiency ratio associated with the germinated chickpea diets compared favorably to that obtained with the casein diet. Protein digestibility decreased as germination time increased. Essential amino acid availability did not change after 24 hr of germination, but small decreases were observed after 48 hr. Protein and starch were studied separately to determine their influence on the observed modifications. No significant changes were found in the concentration of proteins in germinated chickpea even after 72 hr of germination as indicated by densitometry scans of SDS-PAGE patterns. Starch was isolated from intact and germinated chickpeas and characterized by several of its physicochemical properties and its susceptibility to alpha-amylase hydrolysis. Germination increased substantially starch digestibility and modified some of the physico-chemical properties of starch. Scanning electron microscopy (SEM) showed no apparent differences between starches except for a tendency of the germinated chickpea starch to clump. These results suggest that changes in texture, consistency and other physical parameters observed on the germinated chickpea-based products may be attributed mostly to starch.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectChickpea -- Nutrition.en_US
dc.subjectGermination.en_US
dc.subjectFood -- Protein content.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineNutritional Sciencesen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorBerry, James W.en_US
dc.contributor.committeememberReid, Bobby L.en_US
dc.contributor.committeememberMcNamara, Donald J.en_US
dc.contributor.committeememberGoll, Darrell E.en_US
dc.contributor.committeememberBrannon, Patsyen_US
dc.identifier.proquest8814233en_US
dc.identifier.oclc701242743en_US
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.