GENETIC VARIATION IN MITOCHONDRIAL RESPIRATORY METABOLISM IN AVIAN AND MAMMALIAN SPECIES.

Persistent Link:
http://hdl.handle.net/10150/184046
Title:
GENETIC VARIATION IN MITOCHONDRIAL RESPIRATORY METABOLISM IN AVIAN AND MAMMALIAN SPECIES.
Author:
Brown, Daniel Robert
Issue Date:
1987
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Comparative studies documented relationships between mitochondrial respiration and phenotype among genetically divergent animals. Broiler chickens' mitochondria ranked higher than layer chickens' for state 3 respiration (16.1 ± 0.4 μM O₂/mg protein/min), respiratory control ratio (3.5 ± 0.1) and ADP:O ratio (1.05 ± 0.01 μM ADP/μA O) utilizing succinate. Broilers yielded more (P < .05) mitochondrial protein than did layers (7.8 vs. 6.2 ± 0.2 mg/g hepatic tissue). Within-breed mitochondrial activity had no associations with chicken weight or egg production. C57BL/6J mice mitochondria had higher (P < .05) respiratory control ratios (4.06 vs. 3.78 ± 0.09), and C57BL/6J females higher state 3 respiration rates utilizing succinate (strain by sex interaction P < .01), than A/J or BALB/cJ mice mitochondria. BALB/cJ mitochondria had higher (P < .05) ADP:O ratios utilizing pyruvate than C57BL/6J mitochondria (1.58 vs. 1.46 ± 0.04 μM ADP/μA O). Rapid ATP synthesis rates of C57BL/6J female mice were associated generally with reproductive superiority. Inbred mouse body weight was not associated with mitochondrial respiration. Original data confirmed CB6F₁, but not CAF₁, hybrid mice were heterotic for postweaning growth. Hybridization increased (P < .05) mitochondrial mass, but did not enhance respiration. No association existed between growth heterosis and mitochondrial function in hybrid mice. Unexpected sexual dimorphism in mitochondrial respiration existed across mouse strains. Testosterone administration and orchiectomy confirmed female mice exhibited superior organelle activity. Mitochondrial ATP synthesis rates of Holstein cows correlated (+.30 to +.48) with milk production traits of cows and their dams suggested maternal breeding values for milk yield might be improved by considering mitochondrial effects. No breed differences in state 3 respiration (17.7 ± 0.6 μM O2/mg protein/min) or ADP:O ratio with succinate (1.17 ± 0.01 μM ADP/μA O) existed among Angus, Brangus and Hereford cows. Regression analyses of growth traits of cows and their relatives on mitochondrial parameters revealed mainly nonsignificant mitochondrial effects. Significant regression coefficients (-0.43 to -1.05) were found for some cattle growth traits regressed on ADP:O ratios. No mitochondrial complementation occurred between neutrally-combining animal strains. Complementation occurred in mitochondrial mixtures from females of mouse strains with positive combining ability exclusively. Cow mitochondrial mixtures had lower (P < .05) state 3 rates than predicted.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Mitochondria.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Genetics; Graduate College
Degree Grantor:
University of Arizona
Advisor:
DeNise, S. K.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleGENETIC VARIATION IN MITOCHONDRIAL RESPIRATORY METABOLISM IN AVIAN AND MAMMALIAN SPECIES.en_US
dc.creatorBrown, Daniel Roberten_US
dc.contributor.authorBrown, Daniel Roberten_US
dc.date.issued1987en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractComparative studies documented relationships between mitochondrial respiration and phenotype among genetically divergent animals. Broiler chickens' mitochondria ranked higher than layer chickens' for state 3 respiration (16.1 ± 0.4 μM O₂/mg protein/min), respiratory control ratio (3.5 ± 0.1) and ADP:O ratio (1.05 ± 0.01 μM ADP/μA O) utilizing succinate. Broilers yielded more (P < .05) mitochondrial protein than did layers (7.8 vs. 6.2 ± 0.2 mg/g hepatic tissue). Within-breed mitochondrial activity had no associations with chicken weight or egg production. C57BL/6J mice mitochondria had higher (P < .05) respiratory control ratios (4.06 vs. 3.78 ± 0.09), and C57BL/6J females higher state 3 respiration rates utilizing succinate (strain by sex interaction P < .01), than A/J or BALB/cJ mice mitochondria. BALB/cJ mitochondria had higher (P < .05) ADP:O ratios utilizing pyruvate than C57BL/6J mitochondria (1.58 vs. 1.46 ± 0.04 μM ADP/μA O). Rapid ATP synthesis rates of C57BL/6J female mice were associated generally with reproductive superiority. Inbred mouse body weight was not associated with mitochondrial respiration. Original data confirmed CB6F₁, but not CAF₁, hybrid mice were heterotic for postweaning growth. Hybridization increased (P < .05) mitochondrial mass, but did not enhance respiration. No association existed between growth heterosis and mitochondrial function in hybrid mice. Unexpected sexual dimorphism in mitochondrial respiration existed across mouse strains. Testosterone administration and orchiectomy confirmed female mice exhibited superior organelle activity. Mitochondrial ATP synthesis rates of Holstein cows correlated (+.30 to +.48) with milk production traits of cows and their dams suggested maternal breeding values for milk yield might be improved by considering mitochondrial effects. No breed differences in state 3 respiration (17.7 ± 0.6 μM O2/mg protein/min) or ADP:O ratio with succinate (1.17 ± 0.01 μM ADP/μA O) existed among Angus, Brangus and Hereford cows. Regression analyses of growth traits of cows and their relatives on mitochondrial parameters revealed mainly nonsignificant mitochondrial effects. Significant regression coefficients (-0.43 to -1.05) were found for some cattle growth traits regressed on ADP:O ratios. No mitochondrial complementation occurred between neutrally-combining animal strains. Complementation occurred in mitochondrial mixtures from females of mouse strains with positive combining ability exclusively. Cow mitochondrial mixtures had lower (P < .05) state 3 rates than predicted.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectMitochondria.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineGeneticsen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorDeNise, S. K.en_US
dc.contributor.committeememberAllen, Ronald E.en_US
dc.contributor.committeememberRay, Donald E.en_US
dc.contributor.committeememberReid, B. L.en_US
dc.contributor.committeememberMcDaniel, Robert G.en_US
dc.identifier.proquest8712862en_US
dc.identifier.oclc698466011en_US
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