Persistent Link:
http://hdl.handle.net/10150/156918
Title:
Translational Regulation of ICP22 on Other Viral Genes
Author:
Shah, Palak Pankaj
Issue Date:
Dec-2010
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The purpose of this experiment was to observe ICP22's effects on expression of immediate early, early, and late genes of herpes simplex virus-1. This was tested through GFP fluorescence of reporter plasmids after infection with the KOS wild type or the n199 ICP22 null virus. ICP22 suppressed the activity of six of the eight promoters tested, which were ICPO, ICP22/47, ICP27, TK, gC, and VP5. The two viral promoters that did not show suppression were ICP4 and ICP6. We conclude that ICP22 is not a universal repressor, but rather specifically targets certain viral genes and directly regulates them.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Molecular and Cellular Biology
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleTranslational Regulation of ICP22 on Other Viral Genesen_US
dc.creatorShah, Palak Pankajen_US
dc.contributor.authorShah, Palak Pankajen_US
dc.date.issued2010-12-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe purpose of this experiment was to observe ICP22's effects on expression of immediate early, early, and late genes of herpes simplex virus-1. This was tested through GFP fluorescence of reporter plasmids after infection with the KOS wild type or the n199 ICP22 null virus. ICP22 suppressed the activity of six of the eight promoters tested, which were ICPO, ICP22/47, ICP27, TK, gC, and VP5. The two viral promoters that did not show suppression were ICP4 and ICP6. We conclude that ICP22 is not a universal repressor, but rather specifically targets certain viral genes and directly regulates them.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineMolecular and Cellular Biologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
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