Persistent Link:
http://hdl.handle.net/10150/156914
Title:
Neurotrophic Effects of VEGF-B on Dopaminergic Neurons In-Vitro
Author:
Gonzalez, Robert Tomas
Issue Date:
Dec-2010
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Vascular endothelial growth factor B (VEGF-B) has shown potential for therapeutic implementation as a neuroprotective factor in the treatment of neurodegenerative disorders, including Parkinson's disease (PD). The goals of this study were two-fold: (i) to determine the neurotrophic effect of both known VEGF-B isoforms on primary neurite length of dopaminergic (DA) neurons in culture, and (ii) to compare this effect against the neurotrophic effects of other growth factors known to increase neurite outgrowth in culture. Glial-derived neurotrophic factor (GDNF), vascular endothelial growth factor A (VEGF-A165), pigment epithelium derived factor (PEDF), VEGF-B186, and VEGF-B167 were added individually to primary midbrain cultures at various concentrations, with untreated midbrain cultures serving as controls. As expected, GDNF, PEDF, and VEGF-A165 all had a significant effect on primary neurite outgrowth at one or more of their tested concentrations (1ng/mL; 5ng/mL; 1ng/mL and 5ng/mL, respectively) relative to control measurements. VEGF-B186 was shown to have a significant effect on primary neurite outgrowth at all three tested concentrations (5ng/mL; 10 ng/mL; 20 ng/mL), while VEGF-B167 showed a strong neurotrophic trend at both concentrations (5ng/mL; 10ng/mL) that failed to reach significance. Curiously, the effects of GDNF, PEDF, and VEGF-A165 on neurite outgrowth were not as pronounced as those previously observed. However, comparison between both VEGF-B isoforms and the other factors suggests a comparable neurotrophic capacity. We conclude that the growth factor VEGF-B186 increases primary neurite outgrowth of dopaminergic neurons in culture.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Biochemistry and Molecular Biophysics
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleNeurotrophic Effects of VEGF-B on Dopaminergic Neurons In-Vitroen_US
dc.creatorGonzalez, Robert Tomasen_US
dc.contributor.authorGonzalez, Robert Tomasen_US
dc.date.issued2010-12-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractVascular endothelial growth factor B (VEGF-B) has shown potential for therapeutic implementation as a neuroprotective factor in the treatment of neurodegenerative disorders, including Parkinson's disease (PD). The goals of this study were two-fold: (i) to determine the neurotrophic effect of both known VEGF-B isoforms on primary neurite length of dopaminergic (DA) neurons in culture, and (ii) to compare this effect against the neurotrophic effects of other growth factors known to increase neurite outgrowth in culture. Glial-derived neurotrophic factor (GDNF), vascular endothelial growth factor A (VEGF-A165), pigment epithelium derived factor (PEDF), VEGF-B186, and VEGF-B167 were added individually to primary midbrain cultures at various concentrations, with untreated midbrain cultures serving as controls. As expected, GDNF, PEDF, and VEGF-A165 all had a significant effect on primary neurite outgrowth at one or more of their tested concentrations (1ng/mL; 5ng/mL; 1ng/mL and 5ng/mL, respectively) relative to control measurements. VEGF-B186 was shown to have a significant effect on primary neurite outgrowth at all three tested concentrations (5ng/mL; 10 ng/mL; 20 ng/mL), while VEGF-B167 showed a strong neurotrophic trend at both concentrations (5ng/mL; 10ng/mL) that failed to reach significance. Curiously, the effects of GDNF, PEDF, and VEGF-A165 on neurite outgrowth were not as pronounced as those previously observed. However, comparison between both VEGF-B isoforms and the other factors suggests a comparable neurotrophic capacity. We conclude that the growth factor VEGF-B186 increases primary neurite outgrowth of dopaminergic neurons in culture.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineBiochemistry and Molecular Biophysicsen_US
thesis.degree.grantorUniversity of Arizonaen_US
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