Defining the Radiation Sensitizing Activity of the Tubulin Depolymerizing Agent N-Acetyl Colchicinol (NAC)

Persistent Link:
http://hdl.handle.net/10150/156912
Title:
Defining the Radiation Sensitizing Activity of the Tubulin Depolymerizing Agent N-Acetyl Colchicinol (NAC)
Author:
Farmer, Joseph Peter
Issue Date:
Dec-2010
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The goal of this study was to examine the potential for the drug N-acetyl colchicinol (NAC) to be used to increase the efficacy of radiation therapy in the treatment of rectal cancer. In initial studies we defined the maximum non-cytotoxic dose of NAC and demonstrated the ability of NAC to transiently arrest tumor cells in the radiation sensitive G2/M phase of the cell cycle. Using standard clonogenic assays we examined the impact of NAC pretreatment on the cytotoxic activity of the radiomimetic drug Zeocin. Although the unexpectedly high degree of cell death produced by the NAC dose used in these later studies prevented definitive conclusions from being drawn, the results as a whole were encouraging and support further research on the potential of combination therapies employing transient tubulin depolymerizing agents such as NAC and radiation in the treatment of cancer.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Biochemistry and Molecular Biophysics
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleDefining the Radiation Sensitizing Activity of the Tubulin Depolymerizing Agent N-Acetyl Colchicinol (NAC)en_US
dc.creatorFarmer, Joseph Peteren_US
dc.contributor.authorFarmer, Joseph Peteren_US
dc.date.issued2010-12-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe goal of this study was to examine the potential for the drug N-acetyl colchicinol (NAC) to be used to increase the efficacy of radiation therapy in the treatment of rectal cancer. In initial studies we defined the maximum non-cytotoxic dose of NAC and demonstrated the ability of NAC to transiently arrest tumor cells in the radiation sensitive G2/M phase of the cell cycle. Using standard clonogenic assays we examined the impact of NAC pretreatment on the cytotoxic activity of the radiomimetic drug Zeocin. Although the unexpectedly high degree of cell death produced by the NAC dose used in these later studies prevented definitive conclusions from being drawn, the results as a whole were encouraging and support further research on the potential of combination therapies employing transient tubulin depolymerizing agents such as NAC and radiation in the treatment of cancer.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineBiochemistry and Molecular Biophysicsen_US
thesis.degree.grantorUniversity of Arizonaen_US
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