Microsatellite Marker Development in the Western Diamondback (Crotalus Atrox) and Mohave (Crotalus Scutulatus) Rattlesnake Species

Persistent Link:
http://hdl.handle.net/10150/146897
Title:
Microsatellite Marker Development in the Western Diamondback (Crotalus Atrox) and Mohave (Crotalus Scutulatus) Rattlesnake Species
Author:
Bryan, Debra S.
Issue Date:
May-2010
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
We developed novel microsatellite markers for the Crotalus atrox and Crotalus scutulatus species of rattlesnake for future use in population studies. We identified 20 loci from Crotalus atrox and 12 from Crotalus scutulatus to test for amplification and variability. We finally selected loci that showed variation with a number of alleles in diversity panel sample DNA for higher resolution testing. Future directions of this project include assessing allelic richness and variability with fragment analysis, and assessing observed and expected heterozygosity as well as deviations from Hardy-Weignberg equilibrium using the program Genepop 3.4 (Raymond and Rousset, 1995). These assessments will tell us how useful the developed markers will be for future studies in population and conservation genetics.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Biochemistry and Molecular Biophysics
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleMicrosatellite Marker Development in the Western Diamondback (Crotalus Atrox) and Mohave (Crotalus Scutulatus) Rattlesnake Speciesen_US
dc.creatorBryan, Debra S.en_US
dc.contributor.authorBryan, Debra S.en_US
dc.date.issued2010-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractWe developed novel microsatellite markers for the Crotalus atrox and Crotalus scutulatus species of rattlesnake for future use in population studies. We identified 20 loci from Crotalus atrox and 12 from Crotalus scutulatus to test for amplification and variability. We finally selected loci that showed variation with a number of alleles in diversity panel sample DNA for higher resolution testing. Future directions of this project include assessing allelic richness and variability with fragment analysis, and assessing observed and expected heterozygosity as well as deviations from Hardy-Weignberg equilibrium using the program Genepop 3.4 (Raymond and Rousset, 1995). These assessments will tell us how useful the developed markers will be for future studies in population and conservation genetics.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineBiochemistry and Molecular Biophysicsen_US
thesis.degree.grantorUniversity of Arizonaen_US
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