Persistent Link:
http://hdl.handle.net/10150/146852
Title:
A Dynamic Cell Culture System
Author:
Balagopal, Tulika C.; Cheng, Xiao Lucy; Gamboa, Jessica; Harper, John; McPheeters, Sheridan; Notheis, Bryce
Issue Date:
May-2010
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The following includes information regarding the design, construction, and initial testing of A Dynamic Cell Culture System. This project has been undertaken in order to provide the Wu Lab with a tool that will allow a method to more accurately simulate human vasculature for research purposes by applying pulsatile shear, tensile, and normal pressures. This is accomplished by utilizing upper and lower Chambers between which reside tissue scaffolds (composed of silk and elastin) which are seeded with cells. A high flow-rate pump is used to pass cell culture media over the scaffolds in order to create shear. Solenoid valves are used in conjunction with pressure sensors to cause pressure to build up in one Chamber, causing both normal and tensile stresses in the scaffolds. At this time, the system has been fully constructed and has undergone only initial testing, the results of which showed that more work on sealing the Chamber and tube connections is necessary.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Biosystems Engineering
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleA Dynamic Cell Culture Systemen_US
dc.creatorBalagopal, Tulika C.en_US
dc.creatorCheng, Xiao Lucyen_US
dc.creatorGamboa, Jessicaen_US
dc.creatorHarper, Johnen_US
dc.creatorMcPheeters, Sheridanen_US
dc.creatorNotheis, Bryceen_US
dc.contributor.authorBalagopal, Tulika C.en_US
dc.contributor.authorCheng, Xiao Lucyen_US
dc.contributor.authorGamboa, Jessicaen_US
dc.contributor.authorHarper, Johnen_US
dc.contributor.authorMcPheeters, Sheridanen_US
dc.contributor.authorNotheis, Bryceen_US
dc.date.issued2010-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe following includes information regarding the design, construction, and initial testing of A Dynamic Cell Culture System. This project has been undertaken in order to provide the Wu Lab with a tool that will allow a method to more accurately simulate human vasculature for research purposes by applying pulsatile shear, tensile, and normal pressures. This is accomplished by utilizing upper and lower Chambers between which reside tissue scaffolds (composed of silk and elastin) which are seeded with cells. A high flow-rate pump is used to pass cell culture media over the scaffolds in order to create shear. Solenoid valves are used in conjunction with pressure sensors to cause pressure to build up in one Chamber, causing both normal and tensile stresses in the scaffolds. At this time, the system has been fully constructed and has undergone only initial testing, the results of which showed that more work on sealing the Chamber and tube connections is necessary.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineBiosystems Engineeringen_US
thesis.degree.grantorUniversity of Arizonaen_US
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