The Effect of Alternaria Alternata Proteases on Mouse Airway Epithelial Cell Function and Inflammatory Cell Recruitment

Persistent Link:
http://hdl.handle.net/10150/146565
Title:
The Effect of Alternaria Alternata Proteases on Mouse Airway Epithelial Cell Function and Inflammatory Cell Recruitment
Author:
Gruzinova, Irina Sergeevna
Issue Date:
May-2010
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
A murine model has been utilized to investigate the acute effect of Alternaria alternata proteases on airway epithelial cell function and inflammatory cell recruitment. Alternaria exposure and sensitization is associated with the development of asthma in humans and mice, and proteases may be an important component contributing to this effect. Balb/c mice were exposed to Alternaria culture filtrate or filtrate that had been treated either with AEBSF, a serine protease inhibitor, or by heat inactivation to inactivate proteases. Cytokine production was assessed by enzyme-linked immunosorbent assay (ELISA) and real-time quantitative polymerase chain reaction (RT-qPCR); inflammatory cell recruitment was analyzed by lung histology and BALF cytology; and mucin and collagen production was evaluated by lung histology and RT-qPCR. Alternaria serine proteases have been found to play an important role in the induction of inflammatory cytokines and associated inflammatory cell recruitment, while other proteases and allergenic proteins contribute to cytokine production and inflammation less significantly. Mild goblet cell metaplasia was observed in all mice exposed to Alternaria filtrate. There was no observed increase in collagen deposition.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Veterinary Sciences
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleThe Effect of Alternaria Alternata Proteases on Mouse Airway Epithelial Cell Function and Inflammatory Cell Recruitmenten_US
dc.creatorGruzinova, Irina Sergeevnaen_US
dc.contributor.authorGruzinova, Irina Sergeevnaen_US
dc.date.issued2010-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractA murine model has been utilized to investigate the acute effect of Alternaria alternata proteases on airway epithelial cell function and inflammatory cell recruitment. Alternaria exposure and sensitization is associated with the development of asthma in humans and mice, and proteases may be an important component contributing to this effect. Balb/c mice were exposed to Alternaria culture filtrate or filtrate that had been treated either with AEBSF, a serine protease inhibitor, or by heat inactivation to inactivate proteases. Cytokine production was assessed by enzyme-linked immunosorbent assay (ELISA) and real-time quantitative polymerase chain reaction (RT-qPCR); inflammatory cell recruitment was analyzed by lung histology and BALF cytology; and mucin and collagen production was evaluated by lung histology and RT-qPCR. Alternaria serine proteases have been found to play an important role in the induction of inflammatory cytokines and associated inflammatory cell recruitment, while other proteases and allergenic proteins contribute to cytokine production and inflammation less significantly. Mild goblet cell metaplasia was observed in all mice exposed to Alternaria filtrate. There was no observed increase in collagen deposition.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineVeterinary Sciencesen_US
thesis.degree.grantorUniversity of Arizonaen_US
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