Persistent Link:
http://hdl.handle.net/10150/146253
Title:
Electroporation of BmOR Promoters in Manduca Sexta Antennae
Author:
Pacheco, Samantha
Issue Date:
May-2010
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Electroporation is a technique that employs electrical pulses to open pores in a cells membrane to allow DNA to enter and be transcribed using the cells machinery. A Bombyx mori odorant receptor (BmOr) promoter will be introduced to the antennae of Manduca sexta pupa using this technique. Vectors containing a BmOr promoter and TagRFP were constructed using pUC19 as the base plasmid by sequential ligations of first the promoter and then TagRFP using common restriction sites. After ligation the vectors were checked by restriction digest to extract and check the size of the insert and by sequencing the insert and comparing to the expected sequence. Vectors BmOR46-TagRFP-pUC19, BmOR41-TagRFP-pUC19, BmOR27-TagRFP-pUC19 were successfully created and are usable in electroporation experiments as demonstrated by successful electroporation of explanted antennal tissue. Future directions include use of GFP instead of TagRFP as the reporter gene and addition of RNAi to the vector to affect development through loss-of-function of a protein for the purpose of identifying some of the roles specific proteins play in olfactory development. Electroporation will be performed in live stage 4 pupa antenna instead of explants to allow for development of the antenna after introduction of the vector.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Molecular and Cellular Biology
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleElectroporation of BmOR Promoters in Manduca Sexta Antennaeen_US
dc.creatorPacheco, Samanthaen_US
dc.contributor.authorPacheco, Samanthaen_US
dc.date.issued2010-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractElectroporation is a technique that employs electrical pulses to open pores in a cells membrane to allow DNA to enter and be transcribed using the cells machinery. A Bombyx mori odorant receptor (BmOr) promoter will be introduced to the antennae of Manduca sexta pupa using this technique. Vectors containing a BmOr promoter and TagRFP were constructed using pUC19 as the base plasmid by sequential ligations of first the promoter and then TagRFP using common restriction sites. After ligation the vectors were checked by restriction digest to extract and check the size of the insert and by sequencing the insert and comparing to the expected sequence. Vectors BmOR46-TagRFP-pUC19, BmOR41-TagRFP-pUC19, BmOR27-TagRFP-pUC19 were successfully created and are usable in electroporation experiments as demonstrated by successful electroporation of explanted antennal tissue. Future directions include use of GFP instead of TagRFP as the reporter gene and addition of RNAi to the vector to affect development through loss-of-function of a protein for the purpose of identifying some of the roles specific proteins play in olfactory development. Electroporation will be performed in live stage 4 pupa antenna instead of explants to allow for development of the antenna after introduction of the vector.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineMolecular and Cellular Biologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.