Recombination of Two RNA Viruses: Red Clover Necrotic Mosaic Virus and Carnation Ringspot Virus

Persistent Link:
http://hdl.handle.net/10150/146250
Title:
Recombination of Two RNA Viruses: Red Clover Necrotic Mosaic Virus and Carnation Ringspot Virus
Author:
Wang, Andrew C.
Issue Date:
May-2010
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
In this research project, two plant RNA viruses, Red clover necrotic mosaic virus (RCNMV) and Carnation Ringspot Virus (CRSV) were studied and two recombinants were created using RNA1 of RCNMV and RNA2 of CRSV (R1+C2) for one recombinant and RNA2 of RCNMV and RNA1 of CRSV (C1+R2) for the other recombinant. Nicotiana clevelandii and Nicotiana benthamiana were inoculated with the wild types and the two recombinants. The tissues of the plants were extracted for total RNA and a reverse transcription polymerase chain reaction was performed on the total RNA. Using specific primers, the RCNMV wild type from the 12/1/09 total RNA extraction showed the presence of both R1 and R2, while the CRSV wild type from the 12/1/09 total RNA extraction also showed the presence of both C1 and C2. The recombinant C1+R2 from the 12/8/09 total RNA extraction showed the presence of both C1 and R2 and the recombinant of R1+C2 from the 12/1/09 total RNA extraction also showed the presence of both R1 and C2. The next step in this study would be to clone the terminal ends to find out where exactly recombination occurs.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Molecular and Cellular Biology
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleRecombination of Two RNA Viruses: Red Clover Necrotic Mosaic Virus and Carnation Ringspot Virusen_US
dc.creatorWang, Andrew C.en_US
dc.contributor.authorWang, Andrew C.en_US
dc.date.issued2010-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractIn this research project, two plant RNA viruses, Red clover necrotic mosaic virus (RCNMV) and Carnation Ringspot Virus (CRSV) were studied and two recombinants were created using RNA1 of RCNMV and RNA2 of CRSV (R1+C2) for one recombinant and RNA2 of RCNMV and RNA1 of CRSV (C1+R2) for the other recombinant. Nicotiana clevelandii and Nicotiana benthamiana were inoculated with the wild types and the two recombinants. The tissues of the plants were extracted for total RNA and a reverse transcription polymerase chain reaction was performed on the total RNA. Using specific primers, the RCNMV wild type from the 12/1/09 total RNA extraction showed the presence of both R1 and R2, while the CRSV wild type from the 12/1/09 total RNA extraction also showed the presence of both C1 and C2. The recombinant C1+R2 from the 12/8/09 total RNA extraction showed the presence of both C1 and R2 and the recombinant of R1+C2 from the 12/1/09 total RNA extraction also showed the presence of both R1 and C2. The next step in this study would be to clone the terminal ends to find out where exactly recombination occurs.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineMolecular and Cellular Biologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
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