Effect of vesicle size on the photoinduced destabilization of two-component vesicles of dioleoylphosphatidylethanolamine and polymerizable 1,2-bis(10-(sorbyloxy)decanoyl);-sn-glycero-3-phosphatidylcholine.

Persistent Link:
http://hdl.handle.net/10150/144638
Title:
Effect of vesicle size on the photoinduced destabilization of two-component vesicles of dioleoylphosphatidylethanolamine and polymerizable 1,2-bis(10-(sorbyloxy)decanoyl);-sn-glycero-3-phosphatidylcholine.
Author:
Lamparski, Henry.
Issue Date:
1990
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The polymerization of two-component vesicles consisting of the nonpolymerizable lipid dioleolylphosphatidylethanolamine (DOPE) and polymerizable 1,2-bis(10-(sorbyloxy)decanoyl) -sn-glycero-3-phosphatidylcholine (SorbPC) resulted in phase separation of the lipids into polymeric and monomeric domains. Since the nonpolymerizable phosphatidylethanolamine can assume a nonlamellar phase, the polymerization induced phase separation led to vesicle destabilization with concomitant release of aqueous contents. Oligolamellar vesicles of SorbPC/DOPE (1:3) having an average diameter of 275 +/$-$ 65 nm showed 25-30% leakage of the fluorophore calcein while unilamellar vesicles of the same lipid concentration and an average diameter of 125 +/$-$ 15 nm did not show leakage of dye during photopolymerization. The photoinduced destabilization required three processes: (a) lateral phase separation of the membrane's components driven by the photopolymerization; (b) the ability of the nonpolymerizable lipid to assume a nonlamellar phase as enriched domains are formed; and (c) bilayer contact between lamellae. These results are discussed in terms of a model for membrane destabilization that requires intralamellar interaction.
Type:
text; Thesis-Reproduction (electronic)
LCSH Subjects:
Chemistry, Organic
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Chemistry; Graduate College
Degree Grantor:
University of Arizona
Committee Chair:
O'Brien, David F.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleEffect of vesicle size on the photoinduced destabilization of two-component vesicles of dioleoylphosphatidylethanolamine and polymerizable 1,2-bis(10-(sorbyloxy)decanoyl);-sn-glycero-3-phosphatidylcholine.en_US
dc.creatorLamparski, Henry.en_US
dc.contributor.authorLamparski, Henry.en_US
dc.date.issued1990en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe polymerization of two-component vesicles consisting of the nonpolymerizable lipid dioleolylphosphatidylethanolamine (DOPE) and polymerizable 1,2-bis(10-(sorbyloxy)decanoyl) -sn-glycero-3-phosphatidylcholine (SorbPC) resulted in phase separation of the lipids into polymeric and monomeric domains. Since the nonpolymerizable phosphatidylethanolamine can assume a nonlamellar phase, the polymerization induced phase separation led to vesicle destabilization with concomitant release of aqueous contents. Oligolamellar vesicles of SorbPC/DOPE (1:3) having an average diameter of 275 +/$-$ 65 nm showed 25-30% leakage of the fluorophore calcein while unilamellar vesicles of the same lipid concentration and an average diameter of 125 +/$-$ 15 nm did not show leakage of dye during photopolymerization. The photoinduced destabilization required three processes: (a) lateral phase separation of the membrane's components driven by the photopolymerization; (b) the ability of the nonpolymerizable lipid to assume a nonlamellar phase as enriched domains are formed; and (c) bilayer contact between lamellae. These results are discussed in terms of a model for membrane destabilization that requires intralamellar interaction.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subject.lcshChemistry, Organicen_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineChemistryen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.chairO'Brien, David F.en_US
dc.identifier.proquest1339901en_US
dc.identifier.oclc708396070en_US
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