Development of in vitro primary cell cultures from the penaeid shrimp, Penaeus stylirostris and Penaeus vannamei and evaluation of a potential application.

Persistent Link:
http://hdl.handle.net/10150/144635
Title:
Development of in vitro primary cell cultures from the penaeid shrimp, Penaeus stylirostris and Penaeus vannamei and evaluation of a potential application.
Author:
Luedeman, Rene Annette.
Issue Date:
1990
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Studies have been carried out to improve the methods for producing primary cell cultures from penaeid shrimp and to make these procedures routine and practical. Using the methods developed, monolayers of primary cultures of ovarian epithelioid cells from Penaeus stylirostris and P. vannamei were routinely obtained with 60 to 80% confluences within a two day period. A variety of commercially available synthetic tissue culture media and media supplements were tested. It was found that Grace's Insect Medium supplemented with hybridoma quality fetal bovine serum provided the best results. Different culture conditions were tested and it was found that the optimum conditions for shrimp cell growth was a 25-28$\sp\circ$C temperature range with a normal atmosphere. Efforts to infect these primary shrimp ovarian cell cultures from P. stylirostris with infectious hypodermal and hematopoietic necrosis virus (IHHNV) provided inconclusive results.
Type:
text; Thesis-Reproduction (electronic)
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Nutrition and Food Science; Graduate College
Degree Grantor:
University of Arizona
Committee Chair:
Lightner, Donald V.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleDevelopment of in vitro primary cell cultures from the penaeid shrimp, Penaeus stylirostris and Penaeus vannamei and evaluation of a potential application.en_US
dc.creatorLuedeman, Rene Annette.en_US
dc.contributor.authorLuedeman, Rene Annette.en_US
dc.date.issued1990en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractStudies have been carried out to improve the methods for producing primary cell cultures from penaeid shrimp and to make these procedures routine and practical. Using the methods developed, monolayers of primary cultures of ovarian epithelioid cells from Penaeus stylirostris and P. vannamei were routinely obtained with 60 to 80% confluences within a two day period. A variety of commercially available synthetic tissue culture media and media supplements were tested. It was found that Grace's Insect Medium supplemented with hybridoma quality fetal bovine serum provided the best results. Different culture conditions were tested and it was found that the optimum conditions for shrimp cell growth was a 25-28$\sp\circ$C temperature range with a normal atmosphere. Efforts to infect these primary shrimp ovarian cell cultures from P. stylirostris with infectious hypodermal and hematopoietic necrosis virus (IHHNV) provided inconclusive results.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineNutrition and Food Scienceen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.chairLightner, Donald V.en_US
dc.contributor.committeememberGerba, Charles P.en_US
dc.identifier.proquest1339902en_US
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